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Here's original product copy for 5-Amino-1MQ under the Perfect Profile Peptides brand:
5-Amino-1MQ (5-Amino-1-Methylquinolinium) | Research Compound
5-Amino-1MQ is a synthetic methylquinolinium derivative developed as a selective inhibitor of nicotinamide N-methyltransferase (NNMT), a cytosolic enzyme that catalyses methyl transfer from S-adenosyl methionine (SAM) to nicotinamide and related substrates. By interfering with NNMT catalytic activity, 5-Amino-1MQ influences levels of methylated nicotinamide derivatives and related metabolites, making it a valuable molecular tool for investigating the downstream consequences of NNMT inhibition across metabolic research contexts.
NNMT is expressed across multiple tissue types including liver, adipose tissue, and kidney, and plays a significant role in methyl donor balance and NAD⁺ salvage pathway regulation. In preclinical biochemical settings, its inhibition by compounds such as 5-Amino-1MQ produces measurable alterations in intracellular metabolic signalling, shifts in methyl donor availability, and downstream changes in bioenergetic pathways.
Research Applications
5-Amino-1MQ is employed as a molecular probe across a range of metabolism and enzymology research contexts, including studies into NNMT function and regulation, metabolic flux and methylation dynamics, NAD⁺ salvage pathway activity, and the downstream effects of NNMT inhibition on gene expression and metabolite profiling. It is also used in cellular assays examining metabolic phenotype changes and in ex vivo or animal models for mechanistic investigation of metabolic regulation in tissues such as adipose and liver. Appropriate dose-response controls and vehicle controls are recommended to exclude non-specific effects in in vitro systems.
Reconstitution
Dissolve in sterile water, dilute acid (such as 0.1N HCl), or a compatible polar solvent such as DMSO, depending on assay requirements — weigh under dry, inert conditions and avoid moisture uptake. Filter sterilise through a 0.22 µm membrane if required prior to use. Monitor for precipitation or pH shifts before use in sensitive assays, as the ionic character of this compound means buffer composition, ionic strength, and pH can influence its behaviour. Once prepared, aliquot stock solutions and store at −20°C or below. Avoid light exposure, elevated temperatures, and prolonged aqueous storage, as these may degrade or oxidise the compound.
Important Notice
All products supplied by Perfect Profile Peptides are intended strictly for in vitro and laboratory research use only. They are not suitable for human or veterinary administration and should be handled in accordance with standard laboratory safety protocols.
Here's original product copy for 5-Amino-1MQ under the Perfect Profile Peptides brand:
5-Amino-1MQ (5-Amino-1-Methylquinolinium) | Research Compound
5-Amino-1MQ is a synthetic methylquinolinium derivative developed as a selective inhibitor of nicotinamide N-methyltransferase (NNMT), a cytosolic enzyme that catalyses methyl transfer from S-adenosyl methionine (SAM) to nicotinamide and related substrates. By interfering with NNMT catalytic activity, 5-Amino-1MQ influences levels of methylated nicotinamide derivatives and related metabolites, making it a valuable molecular tool for investigating the downstream consequences of NNMT inhibition across metabolic research contexts.
NNMT is expressed across multiple tissue types including liver, adipose tissue, and kidney, and plays a significant role in methyl donor balance and NAD⁺ salvage pathway regulation. In preclinical biochemical settings, its inhibition by compounds such as 5-Amino-1MQ produces measurable alterations in intracellular metabolic signalling, shifts in methyl donor availability, and downstream changes in bioenergetic pathways.
Research Applications
5-Amino-1MQ is employed as a molecular probe across a range of metabolism and enzymology research contexts, including studies into NNMT function and regulation, metabolic flux and methylation dynamics, NAD⁺ salvage pathway activity, and the downstream effects of NNMT inhibition on gene expression and metabolite profiling. It is also used in cellular assays examining metabolic phenotype changes and in ex vivo or animal models for mechanistic investigation of metabolic regulation in tissues such as adipose and liver. Appropriate dose-response controls and vehicle controls are recommended to exclude non-specific effects in in vitro systems.
Reconstitution
Dissolve in sterile water, dilute acid (such as 0.1N HCl), or a compatible polar solvent such as DMSO, depending on assay requirements — weigh under dry, inert conditions and avoid moisture uptake. Filter sterilise through a 0.22 µm membrane if required prior to use. Monitor for precipitation or pH shifts before use in sensitive assays, as the ionic character of this compound means buffer composition, ionic strength, and pH can influence its behaviour. Once prepared, aliquot stock solutions and store at −20°C or below. Avoid light exposure, elevated temperatures, and prolonged aqueous storage, as these may degrade or oxidise the compound.
Important Notice
All products supplied by Perfect Profile Peptides are intended strictly for in vitro and laboratory research use only. They are not suitable for human or veterinary administration and should be handled in accordance with standard laboratory safety protocols.